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Image Search Results
Journal: Scientific Reports
Article Title: Cell Assembly in Self-foldable Multi-layered Soft Micro-rolls
doi: 10.1038/s41598-017-17403-0
Figure Lengend Snippet: Encapsulation and incubation of multiple mammalian cells inside micro-rolls. ( a ) Time-lapse schematic and optical images illustrating the cell encapsulation. Once EDTA solution is added, the films transform and encapsulate the cells suspended on the film surface. ( b ) Confocal reconstructed and cross-sectional (b-1, b-2, b-3) images of cell-laden micro-rolls where ρ is approximately 20 μm. The silk fibroin layers in the micro-rolls and encapsulated cells were labelled with Qdot 655 and calcein, respectively. ( c ) A batch release of films produced an array of cell-containing micro-rolls. ( d ) Confocal cross-sectional images of cell-laden micro-rolls where ρ is approximately 40 μm. ( e ) The number of encapsulated cells inside micro-rolls with l = 400 μm (red dots), 800 μm (blue dots) with respect to the cell density of suspended cells. ( f ) Phase-contrast and reconstructed confocal 3D images of cells inside micro-rolls with ρ = 40 μm after 24 h incubation. HEK cells in micro-rolls formed cell fibres (f-1). By contrast, CHO cells in micro-rolls formed hollow structures (f-2, f-3). The enlarged confocal image on the right shows a cross-sectional view of a hollow structure. ( g ) Handling 400 μm-long cell-laden micro-rolls in culture medium. The micro-rolls can be withdrawn, relocated, and ejected with a picolitre flow using glass capillaries. ( h ) Micrograph of assembled cell-laden micro-rolls in culture medium. Scale bars: 100 μm in ( a – g ), 1 mm in ( f ).
Article Snippet: Chinese hamster ovary (CHO),
Techniques: Encapsulation, Incubation, Produced